Ethnobotanical profiles of wild edible plants recorded from Mongolia by Yunatov during 1940-1951.

Mongolian traditional botanical knowledge has been rarely researched concerning the ethnobotany theory and methodology in the last six decades (Pei in Acta Botanica Yunnanica 135-144, 1988, as reported (Martin in Ethnobotany: A methods manual, Chapman and Hall, 1995)). However, most of the known literature of indigenous knowledge and information regarding the use of local wild plants among Mongolian herders was first documented by several botanical research of Russian researchers in Mongolia through the 1940s and 1950s. One of the most comprehensive works was completed by A. A. Yunatov (1909-1967), which is known as "Fodder Plants of Pastures and Hayfields of the People's Republic of Mongolia" (FPM). Yunatov's research sampled forage plants in Mongolia from 1940 to 1951 and subsequently published a study in 1954. The original transcript of FPM was later translated into Chinese and Mongolian (Cyrillic alphabet) during 1958 and 1968. In addition to morphological characteristics, distribution, habitat, phenology, palatability, and nutrition of forage plants, Yunatov`s record collected local names, the folk understanding and evaluation of the forage, as well as other relevant cultural meanings and the use of local wild plants (collected from the wild as opposed to cultivated plants) in FPM through interviews. The book contains the most precious records created in the 1940s and 1950s on folk knowledge of the Mongolians' wild plants in Mongolia. It was composed of 8 chapters and 351 pages in total. The fifth chapter of FPM, entitled "The systematic overview of forage plants," making up 272 pages (77.49% of the total page counts). The order and content of the book-oriented along with profiles of specific plants. Yunatov collected detailed information on plants, such as the local name, morphology, distribution, habitats, ecological characteristics, and phenology. He also discussed the palatability of livestock, particular forage use, other usages, and chemical composition. Through careful reading and understanding of all three versions of the book (in Russian, Chinese, and Mongolian (Cyrillic alphabet)), the FPM-listed information of edible plants was categorized using ethnobotanical dependent analysis. The list of edible plants was ranked based on purposes and ethnobotanical inventories as per methodology and analysis used in the ethnobotany research. FPM listed 35 species are part of 15 families and 25 genera of wild edible plants. Most species belong to Liliaceae and Allium. Naturally grown grain and some food substitutes (plants that could be used as substitutions for typical food) come from the starchy organs, such as seeds, bulbs, roots, and rhizomes of 12, accounting for 34.28% of all species. Wild vegetables come from the parts of a young plant, tender leaves, young fruits, lower leg of stems, and bulbs of 9 species, accounting for 25.71% of all species. There are only three species of wild fruits, accounting for 8.57% of all edible plant species. Tea substitutes consist of leaves, roots, follicle, and aboveground parts of 8 wild plant species, accounting for 22.85% of all species. Seasonings from the wild were made of the elements such as seeds, rhizomes, tender leaves of 7 species, accounting for 20.00% of all species (Fig,8). Similarities and differences are noticeable in utilizing wild edible plants among Mongolian populations living in Mongolia and Inner Mongolia. Six species of wild edible plants listed in FPM have been proven to be collected and consumed by Mongolians from the Genghis Khan era in the twelfth century to the present day. This proved that the Mongolians have a tradition of recognizing and utilizing wild plants, demonstrating historical and theoretical value. Seven species of plants mentioned in this book were closely correlated to the locals' processing of traditional dairy products, meat, and milk food. Yunatov was not an ethnobotanist, but his accurate documentation of interviews and surveys with Mongolians represents valuable information about the collection and consumption of local wild plants during 1940-1951 in Mongolia. His research mission meant to focus on forage grass, the feed plant that sustained livestock, while he also recorded plants consumed by humans. His records on the edible parts and intake methods of some plants are incomplete. Still, it provided ethnobotanical materials of a remarkable scientific value and a living history of ethnobotany in Mongolian regions. Even by today`s standards, it will be challenging to obtain first-hand information of the richness and to the extent of Yunatov's research.

Wild food plants and fungi sold in the markets of Luang Prabang, Lao PDR.

BACKGROUND: Open air markets hold an important position for ethnobiologists. In Southeast Asia, they are seriously understudied, in spite of their incredible biocultural diversity. In order to fill this gap we recorded plants and fungi sold in the open air markets of Luang Prabang, Lao PDR. METHODS: The markets were visited 38 times in four seasons: the dry season, early monsoon, mid-monsoon, and end-of-monsoon, at least 8 times per season. All items were photographed and voucher specimens were collected. Fungi were identified using DNA barcoding techniques. RESULTS: We recorded 110 species of wild edible plants and 54 species of fungi, including 49 wild-collected species. The sold plants included 86 species of green vegetables, 18 species of fruits and 3 species of flowers. Products from woody species constitute around half of all taxa sold. These include the young shoots of tree leaves, which are used for salads-an interesting feature of Lao cuisine. A large number of extremely rare Russula, with no reference sequences represented in databases or even species unknown to science is present on sale in the markets. CONCLUSIONS: Luang Prabang markets are some of the richest in species of wild edible plants and fungi in Asia, and indeed in the whole world. It is worth pointing out the exceptionally long list of wild edible mushrooms which are sold in Luang Prabang (and probably elsewhere in Laos). We view the Morning Market of Luang Prabang as a cultural treasure that unites the traditions of eating a large number of living species with very diverse flora and fauna. Measures should be taken to strike a balance between local foraging traditions and nature conservation priorities.

Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using a real-time PCR assay.

BACKGROUND: As a result of similar appearances between edible and poisonous plants, 42 patients have ingested poisonous plants from 2013 to 2017 in Korea. We have developed species-specific primer sets of three of edible and poisonous plants sets (Ligularia fischeri & Caltha palustris, Artemisia annua & Ambrosia artemisiifolia and Hemerocallis fulva & Veratrum maackii) for distinguishing both plants using a real-time polymerase chain reaction assay. RESULTS: The efficiencies of the developed primer sets ranged from 87.8% to 102.0%. The developed primer sets have significant correlation coefficient values between the Ct values and the log DNA concentration for their target species (r2 > 0.99). The cut-off lines as the crossing point values of the limit of quantitation of the target species were determined, and all non-target species were amplified later than the cut-off cycles. Then, the effectiveness of the developed primer sets was evaluated using commercial food products and digested samples with simulated gastric juice. CONCLUSION: All of the developed species-specific primer sets were able to detect target DNA successfully in commercial food products and the digested samples. Therefore, the developed species-specific primer sets in the present study would be useful tools for distinguishing between poisonous plants and edible plants. © 2020 Society of Chemical Industry.

The secret of health in daily cuisine: typical healthy vegetables in local markets in central Myanmar.

BACKGROUND: Central Myanmar is located in the Indo-Burma biodiversity hotspot, and the Bamar people are the main ethnic group, which settled there over 1000 years ago. Despite being the core region of the country, central Myanmar has been ignored in previous ethnobotanical studies. Local healthy foods and knowledge are regarded as treasures for resource development and pharmaceutical drug discovery, and market surveys are a good strategy in ethnobotanical research. Thus, we collected and documented typical vegetables and local knowledge in local markets and then analysed the diversity and local knowledge of these vegetables. MATERIALS AND METHODS: Observations and interviews were used in the field study, and 10 markets and fairs were selected in central Myanmar. A total of 277 vegetable stalls or shops were visited. We compared the local knowledge we collected with selected important and typical herbal books on traditional Myanmar medicine. Quantitative analysis, including frequency of citation (FC), relative frequency of citation (RFC) and use value (UV), was used to assess the diversity and local knowledge of these vegetables. RESULTS: A total of 132 plant taxa from 47 botanical families and 116 genera were collected. Most (106 taxa, 80.3%) of these vegetables were cited by the informants as functional foods that had health benefits, while others were regarded as merely "good for health". The main health function of the vegetables was treating digestive problems. Sixty-four species were recorded in selected herbal books on traditional Myanmar medicine, and forty-seven taxa were not recorded in these books but were nonetheless used as healthy vegetables by local people. Twenty-eight species of vegetables were collected from wild places. CONCLUSION: The diversity and local knowledge of healthy vegetables in central Myanmar were rich. Nevertheless, the diversity of wild vegetables was seemingly relatively low. The possible reason was that we counted only the vegetables that were from entirely wild sources as "wild vegetables". The most frequently cited vegetables were commonly cultivated species, which reflects the fact that plants cultivated on a large scale comprise the major source of vegetables. Some lesser known vegetables could reflect the unique food culture of local people, but most of these were cited only a few times by the interviewees, which caused low UV and RFC rankings for them in the league table. In addition, future research should pay more attention to the food safety of these vegetables.

Ethnobotanical survey of wild edible plants used by Baka people in southeastern Cameroon.

BACKGROUND: Forest inhabitants worldwide, and indigenous people especially, have depended for generations on plants and animals harvested in these ecosystems. A number of Baka hunter-gatherer populations in south-eastern Cameroon became sedentarised in the 1950s, but still rely on hunting and gathering to meet their basic needs. The use of wild edible plants (WEP) by these communities remains largely undocumented. In this study, we record the diversity of WEP used by Baka people in dense rainforests in the Mintom region. The area still contains relatively undisturbed forest expanses, just south of the Dja Biosphere Reserve, one of the most important protected areas in the Congo Basin. METHODS: We conducted two ethnobotanical surveys in 2019 in four villages on the Mintom road. In the first survey, we interviewed a total of 73 individuals to determine WEP usage. In our second survey, we specifically quantified WEP harvested and consumed daily in a number of households over a 2-week period during the major rainy season, when use of forest products is highest. Specimens of all recorded plants were collected and identified at the National Herbarium of Cameroon. RESULTS: We documented 88 plant species and 119 unique species/plant organ/recipes in 1519 different citations. A total of 61 genera and 43 families were noted. Excluding 14 unidentified wild yam species, 17 WEP species had not been reported in previous ethnobotanical surveys of the Baka. Our results showed that cultivated starchy plant foods make up a significant proportion of our study population's daily nutritional intake. CONCLUSIONS: A high diversity of WEP is consumed by the studied Baka communities. The study area is likely to be significant in terms of WEP diversity since 18 out of the 30 "key" non-timber forest products, NTFP, in Cameroon were mentioned. Documentation of the use of WEP by indigenous communities is vital to ensure the continuity of traditional knowledge and future food security.

Plants, food and treatments used by BaKongo tribes in Uíge (northern Angola) to affect the quality and quantity of human breast milk.

BACKGROUND: Angola has one of the highest annual under-five mortality rates in in the world and malnutrition poses a severe problem in the country. This study is the first to focus on the traditional knowledge of plants, foods, and treatments used by the local population in the province of Uíge to affect the quality and quantity of human breast milk, since decades of independence and civil war impeded ethnobotanical studies in this area. METHODS: This study was conducted in eight municipalities in the province of Uíge, Northern Angola in February and March 2018. In 265 semi-structured interviews, 360 informants in 40 rural villages were asked about plants, food, and treatments used to affect the quality and quantity of human breast milk. Additionally, information on child mortality and the duration of breastfeeding were collected. Whenever possible, plant specimens were collected for later identification. To determine the local importance of the collected plants, food, and treatments, the Relative Frequency of Citations was calculated. RESULTS: Most women reported to have no problems with their breast milk production. The duration of breastfeeding meets the recommendations of the World Health Organization (WHO). Across all use categories, 69 plants from 36 plant families, and 21 other foods and treatments could be identified. CONCLUSIONS: The study shows an overview of a variety of plants, foods, and treatments used by mothers as galactagogues, to "clean" or to reduce their breast milk and those which they avoided to use during the lactation period. There is great potential for further research into this traditional knowledge. Also, further analysis of some of the plants could be of interest.

Urban gardening and neglected and underutilized species in Salvador, Bahia, Brazil.

BACKGROUND: Urban agriculture has been evidenced as a food production and environmental sustainability strategy, although it faces many obstacles in Latin American countries. Additionally, in urban areas, low consumption of fruit and greenery is noticeable, along with loss in food diversity, including the neglected and underutilized species (NUS), which involve potential to strengthen local food systems. For this reason, this work has sought to map urban gardens in the city of Salvador, Bahia, Brazil, characterizing their gardeners, and to systematize information regarding food produced and the use of NUS. METHODS: The municipality's urban gardens were mapped and data was collected from the gardeners. The study included two steps: (i) garden localization; (ii) on-site visits for interviews with gardeners and verification of cultivated food, destination of production, availability, and use of NUS. RESULTS: Eighteen active food gardens were located, seventeen of which participated in the study: eight (8) communal (UCG) and nine (9) private (UPG). Respondents were on average 55.76 years old, mostly (52.9%) male, working at UPG (88.9%). Women predominated in the UCG (87.5%), with higher levels of education. For 52.9% of the interviewees, the garden was their main source of income. Food produced at the urban gardens was consumed by 82.4% of the gardeners and their families. In 70.6% of the gardens, production was also sold, while 47.1% donated. During the survey, 59 NUS were found and 76.5% of respondents reported consuming 19 of the species. NUS leaves, fruits, and seeds were found to be eaten raw, boiled, or sautéed in various preparations, especially Coleus amboinicus Lour. (76.5%), Eryngium foetidum L. (35.3%), Talinum fruticosum (L.) Juss., and Pereskia aculeata Mill (both 29.4%). Occurrence and utilization of NUS did not present significant associations with the gardens or gardeners (p > 0.05). CONCLUSIONS: Salvador urban gardens, even in small numbers and without government support, have produced affordable food for the local population, preserved food diversity, and the tradition of NUS cultivation and use. Thus, urban gardens are reaffirmed as relevant spaces that should be included in public policies in order to promote food and nutritional security, biodiversity, and urban environmental sustainability.

Wild edible plants collected and consumed by the locals in Daqinggou, Inner Mongolia, China.

BACKGROUND: Knowledge of wild edible plants is an important part of traditional knowledge. It is closely related to traditional human agriculture, as well as biodiversity. This study aimed to conduct a detailed investigation and evaluation of wild edible plants that are collected and consumed by the Mongolian and Han locals in Daqinggou and to provide valuable data for the development and utilization of plant resources. METHODS: In the 9 site visits to the area of Daqinggou during the period of 2017-2019, the authors used key informant interviews, semistructured interviews, and questionnaires to collect utilization information regarding precollected species of local wild edible plants. By combining the data obtained from 101 key informants, the authors used the Cultural Food Significance Index (CFSI), a quantitative index to evaluate the relative importance of the wild edible plants that were discussed in the aforementioned interviews. RESULTS: The investigation results show that the Mongolian people provided 67 folk names, corresponding to 57 wild plants, and the Han Chinese provided 58 folk names, corresponding to 49 wild plants. A total of 61 edible wild plant species belonging to 29 families and 52 genera were recorded as edible resources for the locals in Daqinggou. The uses include grains, oil and fat resources, vegetables, fruits, beverages, condiments, and snacks. The most commonly reported purpose of wild edible plants is using them as vegetables, followed by using them as beverages and fruits. The most widely used edible parts are fruits, leaves, and other aerial parts. Eating raw and cooked plants are the usual methods of consuming wild edible plants according to the locals. In addition, the CFSI of 61 wild edible plant species shows that 27 species have characteristics of medical food. CONCLUSIONS: The knowledge and experience of naming and consuming wild plants by the Mongolian people and Han Chinese in Daqinggou are an important manifestation of the direct interaction between locals and plants. The CSFI evaluation of the wild edible plants consumed by the locals in Daqinggou establishes the utilization of some wild plants as part of the traditional knowledge of medical food.

Ethnobotanical study on wild edible plants used by three trans-boundary ethnic groups in Jiangcheng County, Pu'er, Southwest China.

BACKGROUND: Dai, Hani, and Yao people, in the trans-boundary region between China, Laos, and Vietnam, have gathered plentiful traditional knowledge about wild edible plants during their long history of understanding and using natural resources. The ecologically rich environment and the multi-ethnic integration provide a valuable foundation and driving force for high biodiversity and cultural diversity in this region. However, little study has uncovered this unique and attractive culture to the world. METHODS: We conducted ethnobotanical survey in 20 villages of Jiangcheng County from 2016 to 2020. Altogether 109 local Dai, Hani, and Yao people were interviewed, and their traditional knowledge about wild edible plants was recorded. Voucher specimens were identified by the authors and deposited in the herbarium of Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences (HITBC). The use value was used as a quantitative index to evaluate the consumption frequency and relative importance of the wild edible plants. The Jaccard index was calculated to assess the usage similarity of different areas. The relationship of age and recognized wild edible plants by different ethnic people was performed by R. RESULTS: A total of 211 wild edible plants, belonging to 71 families and 151 genera, were recorded. These plants were consumed as wild edible vegetables, seasonal fruits, salads, spices, sour condiments, tonic soups, tea substitutes, liquor brewing, or dyeing materials. The use value (UV), current cultivation, market availability, and the quantitative traditional knowledge inheritance situation of these wild edible plants among different generations, were analyzed. Based on the data from the threatened species list of China's higher plants and the IUCN Red List, the food plant list for Asia Elephant, the Subject Database of China Plant, and the calculated UV score, the top 30 most important wild edible plants were selected for further cultivation in some local villages. CONCLUSION: Traditional knowledge of wild edible plants, owned by Dai, Hani, and Yao people in Jiangcheng County, is rich but at risk of being lost among the young generation. Diversified cultivation of wild edible plants by the local communities could be a solution for the sustainable use of natural resources and to conserve the endangered species in this trans-boundary region.

Renegotiating situativity: transformations of local herbal knowledge in a Western Alpine valley during the past 40 years.

BACKGROUND: Mountain environments are fragile socio-ecological systems and the conservation of their biological and cultural diversities- seen as co-evolving, strongly intertwined entities-represents a crucial issue for fostering their sustainability. Very few ethnobiological studies have assessed in the mountainous regions of Europe how local botanical knowledge, which represents a vital portion of the local environmental knowledge (LEK), changes over time, although this may be quintessential for a better understanding of the factors influencing how knowledge and practices are shaped, eroded, or even re-created. METHODS: In the current study, we compared the gathering and use of local medicinal plants in the Upper Sangone Valley, Western Italian Alps, Piedmont (NW Italy) as described in a field study conducted in the mid-seventies and published in 1977 and those arising from field research that we conducted in the spring of 2015 and 2018, during which time ethnobotanical and ethnomycological information concerning both folk medicinal and wild food uses was obtained via 47 in-depth open and semi-structured interviews with community members. RESULTS: In total, one hundred thirty folk taxa represent the past and present medicinal and wild food plant/mushroom heritage of the Sangone Valley: 26 herbal taxa were recorded 40 years ago only; 68 herbal and wild food taxa have been recorded in the current study only; and 36 herbal taxa have been continuously used during the last 40 years. There were no remarkable quantitative differences between the two diachronic medico-ethnobotanical datasets, but the qualitative differences were substantial. The gathering and use of some medicinal plants growing in meadows, forests and higher mountain environments (i.e. Arctostaphylos, Filipendula, Hepatica, Larix, Laserptium, Picea, Polygonatum, Primula, Tussilago and Veronica spp.) disappeared, whereas the collection of plant genera growing in more anthropogenic environments or possibly promoted via popular books and media has been newly introduced (i.e. Aloysia, Apium, Brassica, Crataegus, Epilobium, Fumaria, Geranium, Juniperus, Melissa, Rubus, Rumex, Sedum, Silybum, Taraxacum and Vaccinium spp.). CONCLUSION: The findings show a renegotiation of the situativity that for centuries forged the embeddedness of local communities in their natural environments, probably heavily informed in the past by prevalent pastoralist and forest-centred activities and thus by a deeper knowledge of higher mountain and forest environments. The re-arrangement of a more domestic and more "globalized" herbal knowledge system was possibly inspired by new urban residents, who started to populate the valley at the end of the Seventies, when the original inhabitants abandoned their homes for the urban centres of the Piedmontese plain. The current study suggests that future directions of ethnobiological research should more carefully look at the adaptive capacity of LEK systems.

The best choices: the diversity and functions of the plants in the home gardens of the Tsang-la (Motuo Menba) communities in Yarlung Tsangpo Grand Canyon, Southwest China.

BACKGROUND: Home garden is identified as a kind of small-scale land-use system which is used to manage and cultivate useful plants by local people, and home gardens can provide various plant products and services. Investigating home gardens was regarded as an effective way to understand the biodiversity-related local knowledge and culture of native people in Ethnobiology and Ethnoecology. Home garden is important in less developed and remote areas. The grand canyon of Yarlung Tsangpo is designed as one of the biodiversity hotspots of China, and it is one of the most remote regions of China, because of the rough traffic conditions. The aim of the present study is to collect, record, and document the plants and their local knowledge and functions in the local home gardens, then attempt to answer the question: "why local people selected these plants?" MATERIAL AND METHODS: The study area was in Beibeng Township of Motuo County in the grand canyon of Yarlung Tsangpo. Observation and semi-structure interviews with informed consent were used to collect data in field study. All information was collected and organized, then documented based on "ethno-species" as a fundamental unit. All of the information of local use and knowledge were organized as the list of "use-report" for quantitative analysis, and the local uses of plants were merged into 14 use categories. Frequency of citation (FC), relative frequency of citation (RFC), cultural importance index (CI), and cultural value index (CV) were used in quantitative analysis. Besides, the Jaccard Index was used to compare the similarity in plant species selection among different communities. RESULTS: A total of 78 home gardens in the 9 communities of Beibeng Township were visited, and 196 ethno-species were collected. These ethno-species were identified into 188 Botanical taxa. A total of 87 home garden owners as informants were interviewed in the present study, and they provided 625 use-reports to us. The top 5 important plants were Su-lan-tsao (Dendrobium nobile), Sa-ga (Zingiber officinale), Soe-lu (Capsicum annuum), Snying-pa (Citrus medica), and Kham-pu (Prunus persica), according to the quantitative analysis. The most citied use-category was "vegetable," followed by "ornamental plant," "medicine," and "fruit." The altitude might be the most important impact factor of the plant diversity and composition of home gardens, and the traffic conditions, local terrain, also impact the plant diversity and composition of home gardens. CONCLUSION: In remote areas such as the Yarlung Tsangpo Grand Canyon, the plants in home gardens are important sources of plant products such as foods, herbal medicines, and fibers to support daily lives. The local home gardens in Tsang-la communities had high diversity of plants, and these plants provided many functions and services to support daily lives of local people. Local plant knowledge, including the features, life forms, habits, habitats, and use values of plants, were the summary of the understanding of local people to their surrounding plant worlds. Local people selected appropriate plants to cultivate and manage in their home gardens under the guidance of the local plant knowledge. That is the answer to the question "why local people selected these plants?"

Medicinal dietary plants of the Yi in Mile, Yunnan, China.

BACKGROUND: The Yi is the largest ethnic group in Yunnan Province (China), with a population of five million. The Yi people tend to live in mountainous areas, and their culture includes a unique dietary system for treating and protecting people against illnesses. Medicinal plants occupy an essential place in the Yi diet because they play a key role in health and the prevention and treatment of diseases. However, few studies have addressed these medicinal dietary plants and their importance in the Yi's daily lives. The aim of this study was to (1) investigate the medicinal dietary plants used by the Yi in Mile City, (2) document the traditional knowledge held about these plants, (3) identify species with important cultural significance to the Yi in Mile City, and (4) analyze the special preparation methods and consumption habits of these plants. METHODS: Field investigations were performed in six villages in Mile City, Honghe Hani and Yi Autonomous Prefecture, Yunnan, from July 2017 to May 2018. Information was collected using direct observation, semi-structured interviews, key informant interviews, individual discussions, and focus group discussions. The use value (UV) and frequency of utilization index (FUI) of these plants were analyzed. Plant samples and voucher specimens were collected for taxonomic identification. RESULTS: This study documented 124 species belonging to 62 families and 102 genera. These plants included angiosperms (117 spp.), gymnosperms (3), pteridophytes (2), lichen (1), and fungus (1). The 20 species with the highest UV were noted as being particularly important to the Yi people's daily life in Mile City. The primary medicinal preparation method for plants recorded in the study was decoction. The most commonly used plant parts were fruits and roots. The most frequently used edible parts were fruits, and the most frequently used medicinal parts were roots. The medicinal parts were used to treat diseases such as rheumatism, edemas, kidney deficiency, spleen deficiency, gastritis, parasites, and so on. CONCLUSION: A wide variety of medicinal dietary plants are used by the Yi people in Mile City. Those plants, which have both rich nutritional and medicinal value, occupy an essential part of the Yi dietary and medicine culture. Ethnobotanical surveys of medicinal dietary plants provide a theoretical reference for the conservation and sustainable use of the plant resources and could contribute to the protection of the Yi food culture and traditional medicine in Mile City. In addition, this information provides a sound basis for developing and using Yi ethnic medicine and health products.

Knowledge transmission patterns at the border: ethnobotany of Hutsuls living in the Carpathian Mountains of Bukovina (SW Ukraine and NE Romania).

BACKGROUND: Cross-border research is a novel and important tool for detecting variability of ecological knowledge. This is especially evident in regions recently divided and annexed to different political regimes. Therefore, we conducted a study among Hutsuls, a cultural and linguistic minority group living in Northern and Southern Bukovina (Ukraine and Romania, respectively). Indeed, in the 1940s, a border was created: Northern Bukovina was annexed by the USSR while Southern Bukovina remained part of the Kingdom of Romania. In this research, we aim to document uses of plants for food and medicinal preparations, discussing the different dynamics of Local Ecological Knowledge (LEK) transmission among Hutsuls living in Ukraine and Romania. METHODS: Field research was conducted using convenience and snowball sampling techniques to recruit 31 Hutsuls in Ukraine and 30 in Romania for participation in semi-structured interviews regarding the use of plants for medicinal and food preparation purposes and the sources of such knowledge. RESULTS: The interviews revealed that, despite a common cultural and linguistic background, ethnobotanical knowledge transmission occurs in different ways on each side of the border. Family is a primary source of ethnobotanical knowledge transmission on both sides of the border; however, in Romania, knowledge from other sources is very limited, whereas in Ukraine interviewees reported several other sources including books, magazines, newspapers, the Internet and television. This is especially evident when analysing the wild plants used for medicinal purposes as we found 53 taxa that were common to both, 47 used only in Ukraine and 11 used only in Romania. While Romanian Hutsuls used almost exclusively locally available plants, Ukrainian Hutsuls often reported novel plants such as Aloe vera, Aronia melanocarpa and Elaeagnus rhamnoides. Knowledge related to these plants was transferred by sources of knowledge other than oral transmission among members of the same family. Therefore, this may imply hybridization of the local body of knowledge with foreign elements originating in the Soviet context which has enriched the corpus of ethnobotanical knowledge held by Ukrainian Hutsuls. CONCLUSIONS: While ethnobotanical knowledge among Romanian Hutsuls is mainly traditional and vertically transmitted, among Ukrainian Hutsuls there is a considerable proportion of LEK that is transmitted from other (written and visual) sources of knowledge. This cross-border research reveals that despite a common cultural background, socio-political scenarios have impacted Hutsul ethnobotanical knowledge and its transmission patterns.

Filling the gaps: ethnobotanical study of the Garrigues district, an arid zone in Catalonia (NE Iberian Peninsula).

BACKGROUND: This study has focused on the Garrigues district, one of the most arid regions in Catalonia (NE Iberian Peninsula), which, in general terms, has remained unexplored from the ethnobotanical point of view. This area, of 22,243 inhabitants, comprises 33 municipalities distributed across 1123.12 km2. The natural vegetation is dominated by holm oak forests and maquis called 'garriga', the latter giving its name to the district. During the last few decades, this landscape has been transformed by agricultural activities, nowadays in recession. The main aim of this work was to collect and analyse the ethnoflora of this area in order to fill a gap in the ethnobotanical knowledge in Catalonia. METHODS: The followed methodology was based on semi-structured interviews. The obtained data have been qualitatively and quantitatively analysed and compared with other available ones. RESULTS: Data were gathered from 68 interviews involving 101 informants, whose ages range from 24 to 94, the mean being 73.07. The number of taxa reported in this study was 420, belonging to 99 botanical families. The interviewed informants referred 4715 use reports (UR) of 346 useful taxa, 1741 (36.93) of them corresponding to medicinal uses, 1705 (36.16%) to food uses, and 1269 (26.91%) to other uses. This study has inventoried, apart from individual plant uses, 260 plant mixtures, of which 98 are medicinal and 162 food. In the present study, 849 vernacular names with 116 phonetic variants have been collected, as well, for 410 taxa. The informant consensus factor (FIC) obtained for our interviewees is 0.93, and the ethnobotanicity index is 23.47% for the studied area. Apart from plants belonging to the typical Catalan, Iberian or European ethnofloras, the present work contributes information on some plants from semiarid or arid regions, such as Artemisia herba-alba and Plantago albicans, much rarer in the ethnobotany of the quoted areas. CONCLUSIONS: The results of this study reveal the persistence of ethnobotanical knowledge in the prospected area and the importance of filling the existing gaps in the ethnofloristic sampling of the Catalan territories. The almost complete dataset, now including some arid territories, will allow us to carry out a global analysis and to provide an accurate overview.

Comparative analysis of the skim milk and milk fat globule membrane proteomes produced by Jersey cows grazing pastures with different plant species diversity.

The objective of this experiment was to identify and characterize the bovine milk proteome within the skim milk fraction and milk fat globule membrane (MFGM)-associated fraction from 16 organically certified lactating Jersey cows after a short term of grazing pastures with or without annual forage crops (AFC). Cows were offered a partial mixed ration (∼60% of dry matter intake) and approximately 40% of their total dry matter intake as herbage. Eight cows were offered a cool-season grass-legume herbage (GLH), which included orchardgrass (Dactylis glomerata), timothy (Phleum pratense), Kentucky bluegrass (Poa pratensis), and white clover (Trifolium repens). The other 8 cows were offered the same GLH strip-tilled with the AFC, including oat (Avena sativa), millet (Pennisetum glaucum), teff (Eragrostis tef), buckwheat (Fagopyrum esculentum), and chickling vetch (Lathyrus sativus). Milk samples were collected from each cow during a.m. and p.m. milkings on d 19 to 21 of grazing, and composite milk samples per cow were analyzed for (1) the high-abundance milk protein profile, (2) the skim milk low-abundance protein-enriched proteome, and (3) the MFGM proteome. Of the 443 proteins identified in the skim and MFGM proteomes, 433 were included in statistical analysis, including 68 proteins identified in the skim milk fraction and 365 in the MFGM-associated fraction. Analysis of the skim and MFGM proteomes encompassed unique gene ontology profiles and proportions of functional classifications. In response to diet, αS1-casein as well as 8 low-abundance proteins were present in higher concentration or abundance in milk from cows grazing the GLH strip-tilled with the AFC compared with milk from cows grazing GLH, suggesting that even short-term grazing of pastures including some AFC may affect the milk proteome.

Wild plants and fungi sold in the markets of Yerevan (Armenia).

BACKGROUND: The aim of the study was to record wild plants and fungi sold in the capital of Armenia. This is the first large market survey in the Caucasus region. The area of the Caucasus is characterised by a very high diversity of climates, flora and languages which results in very rich traditions of plant use. METHODS: Interviews were conducted and photos and voucher specimens were taken during multiple visits made over 4 years. We studied 37 locations and 136 people were interviewed. RESULTS: As many as 163 plant species, belonging to 44 families and 110 genera, were recorded on Yerevan markets. This included 148 wild food species, 136 medicinal species, 45 species sold for decoration, 15 species of wood and 9 species of insect repellents. Also 14 wild species of fungi were sold, including 12 food species. CONCLUSIONS: The list of plants sold in the markets of Yerevan is very extensive and diverse, and includes many species of wild fruits, vegetables and medicinal plants, some of them never listed in ethnobotanical directories before. A characteristic feature of this market is a large representation of lacto-fermented products. Some of the species sold in Yerevan have never been reported as human food either in wild edible plant word lists or in ethnobotanical publications, e.g. Angelica tatianae, Ferulago setifolia and Heracleum chorodanum. Fungi are also well represented.

Berries, greens, and medicinal herbs-mapping and assessing wild plants as an ecosystem service in Transylvania (Romania).

BACKGROUND: Wild edible plants as well as medicinal herbs are still widely used natural resources in Eastern Europe that are frequently accessed by the local population. Ethnobotanical studies rarely give insight to the specific ecosystems in which wild food and medicinal plants grow in a spatially explicit way. The present work assesses the potential of different ecosystems to provide wild plants for food and medicinal use based on 37 selected plant species, gives an estimate on the actual use of wild plants, and allows insights into the motivation of local people to collect wild plants. METHODS: A number of interdisciplinary methods were used: participatory stakeholder workshops with experts scoring the provisioning capacity of ecosystem types, GIS for representing results (capacity maps), basic data statistics for actual use assessment, and interviews for analysing motivations. RESULTS: Capacity to provide wild edible plants was assessed highest in broad-leaved forests and wetlands, while for medicinal herbs, orchards were rated best. We could find a multitude of motivations for gathering that could be grouped along four main lines corresponding to major dimensions of well-being (health, habit/tradition, nutrition/income, pleasure/emotional), with health reasons dominating very clearly the range (59% of answers), which can be interpreted as a combination of modern "green" values with a traditional lifestyle. We detected some distinct patterns of motivations between the different social groups analysed with more fundamental needs associated with lower level socio-ecological background. CONCLUSION: This case study provides an example on the importance of wild plants for locals from several points of view. We emphasize the relevance of these local stakeholder views to be included in decision-making and ecosystem management, which can be achieved by the presented workflow for mapping and assessment of ecosystem services which is also compatible with EU-suggested Mapping and Assessment of Ecosystems and their Services (MAES).

Identification and frequency of consumption of wild edible plants over a year in central Tunisia: a mixed-methods approach.

OBJECTIVE: To identify wild plants used as food and assess their frequency of consumption over a year in a region of Tunisia where agriculture is undergoing a major transformation from smallholder farming to an intensive high-input agricultural system. DESIGN: Qualitative ethnobotanical study followed by a survey of women's frequency of consumption of wild plants conducted using FFQ at quarterly intervals. SETTING: Sidi Bouzid governorate of central Tunisia. PARTICIPANTS: Mixed-gender group of key informants (n 14) and focus group participants (n 43). Survey sample of women aged 20-49 years, representative at governorate level (n 584). RESULTS: Ethnobotanical study: thirty folk species of wild edible plants corresponding to thirty-five taxa were identified by key informants, while twenty folk species (twenty-five taxa) were described by focus groups as commonly eaten. Population-based survey: 98 % of women had consumed a wild plant over the year, with a median frequency of 2 d/month. Wild and semi-domesticated fennel (Foeniculum vulgare Mill. and Anethum graveolens) was the most frequently consumed folk species. Women in the upper tertile of wild plant consumption frequency were more likely to be in their 30s, to live in an urban area, to have non-monetary access to foods from their extended family and to belong to wealthier households. CONCLUSIONS: In this population, wild edible plants, predominantly leafy vegetables, are appreciated but consumed infrequently. Their favourable perception, however, offers an opportunity for promoting their consumption which could play a role in providing healthy diets and mitigating the obesity epidemic that is affecting the Tunisian population.

Changes in traditional ecological knowledge of forage plants in immigrant villages of Ningxia, China.

BACKGROUND: Ecological migration serves as an important measure for poverty eradication as well as for the protection, inheritance, and utilization of traditional ecological knowledge. This study investigated and cataloged the traditional forage plant resources and recorded the associated traditional knowledge of immigrant villages in Hongsibu District of Ningxia, China. The diversity of traditional forage plant resources and the changes in associated traditional ecological knowledge were compared among ecological immigrant villages from different emigration areas, with a hope of providing a reference for forage development, the conservation of wild forage plant resources, and the development of regional animal husbandry. METHODS: From March 2018 to May 2019, a field investigation was conducted in six villages in Ningxia. Through the snowball technique, a total of 315 immigrants were interviewed using various methods, including semistructured interviews and key person interviews, which included opportunities for free listing. The changes in the utilization of traditional forage plants were compared between the ecological migrants and the original inhabitants, and the causes underlying the changes were analyzed. In addition, the major forage plant species in the research area were investigated and evaluated. RESULTS: (1) The six investigated villages reported 224 traditional forage plant species that belong to 42 families and 150 genera. Compared with their original living areas, the number of traditional forage plant species used in the immigrant villages decreased with the increase in the relocation distance. (2) The utilization of traditional forage plants varied among the immigrants who moved to Hongsibu District from forest areas, loess hilly areas, and semiarid desertified areas. The smaller the difference was in ecological environment between the immigration and emigration areas, the more the traditional forage plant knowledge had been retained. (3) The diversity and associated knowledge of traditional forage plants retained by ecological migrants are closely correlated to gender, age, education level, and occupation. CONCLUSION: This study revealed that the diversity of traditional forage plants and associated knowledge retained after migration vary among ecological immigrants from different areas; generally, the immigrants that relocated from a closer place retained more ecological knowledge. In the immigrant villages with significantly different natural resources and a long distance from the migrants' original locations, the diversity of traditional forage plants decreased, and the traditional knowledge about forage plants showed signs of being forgotten and abandoned by the younger generation. Therefore, measures are urgently needed to document and protect the forage plant resources and preserve the traditional knowledge of ecological immigrants.

Wild edible plants in Yesilli (Mardin-Turkey), a multicultural area.

BACKGROUND: The Yesilli district (Mardin) is located in the southeastern of Turkey and hosts different cultures. The objective of this study was to record the traditional knowledge of wild edible plants used by indigenous people in Yesilli, where no ethnobotanical studies have been conducted previously. METHODS: An ethnobotanical study was carried out in Yesilli district in March 2017-March 2019 to document the traditional knowledge of wild edible plants. The data were collected by interviewing 62 informants. Additionally, the data were analysed based on the cultural importance index (CI) and factor informant consensus (FIC) to determine the cultural significance of wild edible plants and knowledge of wild edible plants among the informants. RESULTS: We documented 74 wild edible taxa belonging to 31 families and 57 genera in the present study. The richness of the wild edible taxa was highest for vegetables (46 taxa), followed by medicinal plants (17 taxa) and fruit (14 taxa). The most important families were Asteraceae (ten taxa), Rosaceae (seven taxa) and Fabaceae (six taxa). The most culturally important taxa (based on the CI index) were Ficus carica subsp. carica, Lepidium draba, Anchusa strigosa, Rhus coriaria, Glycyrrhiza glabra, Sinapis alba, Gundelia tournefortii, Notobasis syriaca, Onopordum carduchorum, Malva neglecta, Mentha longifolia, Juglans regia and Urtica dioica. The maximum number of use reports was recorded for vegetables (1011). The factor informant consensus index (Fic) varied between 0.95 and 0.98 for preserved vegetables, beverages and spices and processed fruits have the highest Fic (0.99). We reported for the first time the ethnobotanical usage of 12 taxa as food. We also recorded the use of Allium wendelboanum, an endemic species in the study area. CONCLUSION: The obtained data were compared with data from other wild edible and ethnobotanical studies conducted in Turkey and particularly those conducted in eastern Turkey. Furthermore, the data were compared with data from studies conducted in the bordering countries of Iraq and Armenia. The present study reflects the cultural diversity of the region, and it is necessary to conduct more studies since it is thought that this diversity will contribute to the economy. This study will enable the traditional use of wild plants as food sources to be passed on to future generations.